Pharmacognosy and Phytochemistry
Chemistry, biosynthesis, isolation and analysis of secondary metabolites — alkaloids, glycosides, flavonoids, tannins, volatile oils and resins.
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Course contents — unit by unit · PCI NEP 2020 BP205T
Unit 1 · Metabolic Pathways & Biogenetic Studies 10 hrs
The basic metabolic pathways and biosynthesis of secondary metabolites — the shikimic-acid, acetate and amino-acid pathways; the use of radioactive isotopes in biogenetic studies; and pathway-prediction and modern genetic tools such as CRISPR/Cas9.
Unit 2 · Primary Metabolites 12 hrs
Pharmacognostic study of drugs containing primary metabolites — carbohydrates (acacia, agar, tragacanth, honey), proteins and enzymes (gelatin, casein, papain, bromelain, streptokinase), and lipids (castor oil, olive oil, cocoa butter, wool fat, beeswax).
Unit 3 · Secondary Metabolites 12 hrs
Pharmacognostic study of drugs containing secondary metabolites — alkaloids (Vinca, Rauwolfia, Opium), volatile oils (lemongrass, clove, cinnamon), tannins, resins (guggul, asafoetida), glycosides (senna, liquorice, digitalis), and flavonoids and terpenoids.
Unit 4 · Extraction Methods for Medicinal Plants 12 hrs
Conventional extraction — infusion, decoction, maceration, percolation, reflux, distillation and Soxhlet and successive-solvent extraction; and modern methods — supercritical-fluid, microwave-assisted, ultrasonic-assisted, enzyme-assisted and pressurized-liquid extraction.
Unit 5 · Isolation, Identification & Characterization 14 hrs
Separation and isolation techniques (planar, column, preparative TLC and flash chromatography); identification by phytochemical, chromatographic and spectroscopic techniques; TLC/HPTLC fingerprinting and markers; and the screening of alkaloids, glycosides, saponins, tannins, flavonoids and steroids.
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